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. Author manuscript; available in PMC: 2021 Apr 1.
Published in final edited form as: Nat Cell Biol. 2020 Oct 1;22(10):1276–1285. doi: 10.1038/s41556-020-00586-6

Fig. 1: Genome-wide CRISPR screen identified LRRC31 as a radiosensitizing gene.

Fig. 1:

a, Top 12 gRNAs ranked based on read number and FPKM value. Cutoff value for FPKM is 10,000 except KATNA2, which has the second highest read number but a small FPKM value. b, WB analysis of the expression of LRRC31 in 231BR cells transduced with Cas9 and the indicated sgRNAs. c, Clonogenic analysis of 231BR cells with down-regulation of LRRC31 7 days after irradiation (n=3 biologically independent samples). d, Kaplan-Meier survival analysis of mice bearing 231BR tumors with down-regulation of LRRC31 in the brain after irradiation (n=5 animals). e, WB analysis of the expression of LRRC31 in 231BR cells transduced with control vector or lentiviral vector for LRRC31 overexpression. f, Clonogenic analysis of 231BR cells with overexpression of LRRC31 7 days after irradiation (n=3 biologically independent samples). g, Kaplan-Meier survival analysis of mice bearing 231BR tumors with overexpression of LRRC31 in the brain after irradiation (n=5 animals). Blots are representatives of two biologically independent experiments with similar results obtained. Unprocessed immunoblots are shown in Source Data Fig. 1. For c and f, data show the mean ± s.d.. Differences between two different groups were compared using the unpaired, two-tailed Student’s t-test. For d and g, experiments were carried out according to the schematic diagram shown in Extended Data Fig 1c. Statistical calculations for Kaplan-Meier survival analysis are included in Source Data 1.