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. Author manuscript; available in PMC: 2021 Apr 1.
Published in final edited form as: Nat Cell Biol. 2020 Oct 1;22(10):1276–1285. doi: 10.1038/s41556-020-00586-6

Extended Data Fig. 8. Validation of the biological effects of LRRC31 in 231BR cells transduced with doxycycline (DOX)-inducible lentiviral vector.

Extended Data Fig. 8

a, WB analysis of the expression of LRRC31 expression in 231BR cells that were transduced with control vector or DOX-inducible LRRC31 overexpression vector and treated with and without DOX (100 ng/ml). Blot is representative of two biologically independent experiments with similar results obtained. Unprocessed immunoblots are shown in Source Data Extended Data Fig. 8. b–d, DOX-induced overexpression of LRRC31 sensitized cells to irradiation (b), and inhibited cell proliferation (c) and DNA-PK activity (d). Data show the mean ± s.d. (n=3 biologically independent experiments). Statistical analysis was performed using the two-tailed, unpaired Student’s t-test.