Figure 1.

Recapitulation of diastolic dysfunction in patient-specific hypertrophic cardiomyopathy (HCM) induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs). (A) Representative immunostaining image of micropatterned iPSC-CMs, showing aligned sarcomere structure. (B and C) Sarcomeres in single iPSC-CMs were indicated by GFP-actin over-expression, and the sarcomere shortening were captured by live-cell confocal line-scanning. (D–G) Results show shorter diastolic sarcomere length (D), unchanged maximum relaxation sarcomere length (E), slower relaxation velocity (F), and prolonged normalized relaxation duration (G) in all HCM iPSC-CM groups compared with iPSC-CMs by one-way ANOVA (Tukey method). N = 42, 36, 40, and 44 cells in Ctrl, MYH7, MYBPC3, and TNNT2 groups, respectively. For each group, data were generated from two different iPSC lines and three batches of differentiation.