FIGURE 4.

Ageing reduces spread of Ca²⁺ events in hippocampal astrocytes. (a and b) x‐y‐time reconstruction of detected Ca²⁺ events in hippocampal str. radiatum (top) with examples of individual events zoomed in from the boxed regions (bottom) in the adult (a) and old (b) mice. (c–e) Summary of event duration (c), area (maximal projection, d), and volume (e) in the adult (black diamonds) and old (green diamonds) mice. Each point represents an individual event. Horizontal black bar indicates the median. (f) Ca²⁺ event initiation points with the colour‐coded number of events initiated during 10 min recording. (g) The distribution of Ca²⁺ events initiation points according to the number of events initiated per pixel in the adult (black trace) and old (green trace) mice.*p < 0.05; **p < 0.01; Mann–Whitney test