Figure 3.

Temperature sensitivity of resistance to initial bacterial invasion and Eat in daf‐2(e1370). These results are consistent with the possibility that class 2 daf‐2 Eat contributes to resistance to P death. (a) Effects of mutation of daf‐2 on pharyngeal pumping rates in early adulthood at 15˚C, 20˚C, and 25˚C. A reduction in pumping rate was consistently observed in class 2 mutants at 25˚C only, consistent with earlier findings (Gems et al., 1998; Kenyon et al., 1993). n ≥ 10. Star indicates p < 0.01. (b) daf‐2(e1370) had little effect on initial bacterial invasion but suppressed widespread infection at 15˚C, delayed both bacterial invasion and further infection at 20˚C, and fully suppressed bacteria invasion at 25˚C. N = 2 trials