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. 2021 Feb 20;20(3):e13324. doi: 10.1111/acel.13324

Figure 6.

Figure 6

Effect of daf‐12(m20) mutation on daf‐2 Age at 25˚C, and model of the effects of daf‐2 mutations on C.elegans lifespan. (a) P frequency and (b) lifespan of wild type and two class 1 daf‐2 mutants without or with daf‐12(m20) at 25˚C, with internal hatching censored. daf‐12(m20) did not significantly affect p Age in class 1 daf‐2 mutant. N = 2 trials. (c) P frequency and (d, e) lifespan of wild type and class 2 daf‐2(e1370) mutant without or with daf‐12(m20) at 25˚C, with internal hatching censored (d) or not censored (e) after necropsy. daf‐12(m20) did not significantly affect lifespan in class 2 daf‐2 mutant. N = 2 trials. (f) Model for how class 1 and class 2 daf‐2 mutations affect C. elegans lifespan. Thickness of lines from DAF‐2 receptors represent severity of mutant effect. Red stars represent the lesion; in most cases, class 1 and class 2 mutations affect the extracellular and intracellular regions of the DAF‐2 receptor, respectively (Kimura et al., 1997; Patel et al., 2008). Class 1 and class 2 mutations exert differential effects on the two major isoforms of DAF‐16 in the pharynx and the intestine. Dashed lines indicate potential effect