A. Elutriation expt. 2. Repeat of Elutriation experiment 1.
B. Elutriation expt. 3. Repeat of Elutriation experiment 1.
C. Alternative size-scaling experiment. cdc28-as cells were treated with 1NMPP1 to block cell cycle progress without additional manipulations. Samples were taken with time, characterized for cell size and cell cycle distribution, processed for RNA-Seq, and analyzed as in Fig. 2C (Materials and Methods).
D. Control. As panel 3C, but using WT CDC28 cells (not sensitive to 1NMPP1). The Wilcoxon p-values for separation of activators and inhibitors are shown.