Skip to main content
. Author manuscript; available in PMC: 2021 Apr 16.
Published in final edited form as: Mol Cell. 2020 Apr 3;78(2):359–370.e6. doi: 10.1016/j.molcel.2020.03.012

Fig 4. Size Scaling at the Protein Level.

Fig 4.

A. The split-GFP system.

B. Timecourse of GFP-Cln3 fluorescence after addition of 1NMPP1 (scalebar = 5 microns).

C. Quantitation of GFP-Cln3 and Whi3-GFP concentration as function of volume in unbudded cells in timecourse after arrest by 1NMPP1. Volume (arbitrary units) estimated from cross-sectional area of cells.

D, E, F. Western analysis of Cln2, Whi2, and Sic1 abundance after arrest by 1NMPP1. Samples (equal total protein) loaded in duplicate (technical replicate). Lane “C” is an untagged control. Arp7 and tubulin are controls.

See also Fig. S5.