KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| HA Tag recombinant rabbit polyclonal | Invitrogen | 8HCLC |
| TAP Tag rabbit polyclonal | Invitrogen | CAB1001 |
| Anti-Arp7 goat polyclonal | Santa Cruz | Sc-8961 |
| Donkey anti-mouse | LI-COR | IRDye 800Cw |
| Donkey anti-rabbit | LI-COR | IRDye 800Cw |
| Donkey anti-goat | LI-COR | 680RD |
| Chemicals, Peptides, and Recombinant Proteins | ||
| 1NMPP1 (4-Amino-1-tert-butyl-3-(1’-naphthylmethyl)pyrazolo[3,4-d]pyrimidine) | Toronto Research Chemicals | A603003 |
| Low Fluorescence Yeast Nitrogen Base | US Biological Life Sciences | Y2025–01 |
| Yeast Nitrogen Base w/o AA, carb & w/AS | US Biological Life Sciences | Y2025 |
| Yeast Extract | US Biological Life Sciences | Y2010 |
| Peptone-Y | Bio101 | 4018–532 |
| DMSO (dimethylsulfoxide) | J.T. Baker | 9224–01 |
| cOmplete protease inhibitor cocktail | Millipore-Sigma | 11697498001 |
| Bradford dye reagent | BioRad | 5000006 |
| Laemmli protein sample buffer | BioRad | 1610747 |
| Precision Plus Protein Dual Color standards | BioRad | 1610374 |
| HA Tag Recombinant rabbit polyclonal antibody | Invitrogen | 8HCLC |
| Sytox green | Thermo Fisher | S7020 |
| Critical Commercial Assays | ||
| Ovation Universal RNA-Seq System, Custom | NuGen | N/A |
| Deposited Data | ||
| BAM files from RNA-Seq | GEO | GSE145206 |
| Experimental Models: Organisms/Strains | ||
| Yeast Deletion Set, strains BY4741, BY4742 | Horizon (current source) | N/A |
| YC31–1c MATα leu2 ura3 | This work | N/A |
| YC34–1a cln2::CLN2prWHI5 whi5::WHI5prCLN2 | This work | N/A |
| YC18–2a MATα cdc28-as | This work | N/A |
| YC31–2a MATα leu2 ura3 cln2::kanMX whi5::kanMX | This work | N/A |
| YCT01 MATα leu2 ura3 whi5::kanMX | This work | N/A |
| YCT02 MATa leu2 ura3 cln2::kanMX | This work | N/A |
| YCT03a MATα leu2 ura3 whi5::WHI5pr-CLN2 | This work | N/A |
| YC32–2d MATα leu2 ura3 cln2::kanMX whi5::WHI5pr-CLN2 | This work | N/A |
| YC33–1d MATα leu2 ura3 cln2::CLN2pr-WHI5 whi5::kanMX | This work | N/A |
| YCT04 MATα leu2 ura3 cln2::CLN2pr-WHI5 | This work | N/A |
| GZ238 MATa leu2 ura3 | Zhao et al. 2016 | N/A |
| GZ240 MATa | Zhao et al. 2016 | N/A |
| GZ241 MATalpha | Zhao et al. 2016 | N/A |
| BF532–2a cdc28-as 5xGFP11-CLN3 gal1,10::TEF-GFP1–10 mCherry-PEST::LEU2 ade2 his3 ura3 leu2 | This work | N/A |
| BF530–6b cdc28-as SIC1-TAP | This work | N/A |
| BF530–6b cdc28-as WHI2-TAP | This work | N/A |
| CLN2C cdc28-as | This work | N/A |
| WHI3-GFP (BY4741 background) | Cai and Futcher 2013 | N/A |
| Recombinant DNA | ||
| pRS425_TEF1_GFP1–10_IDT | This work | N/A |
| Pap_6GFP_HIS5 | This work | N/A |
| pRS426_GAL1_sc5xGFP11_CLN3 | This work | N/A |
| Software and Algorithms | ||
| R Programming language | http://www.rproject.org | N/A |
| Custom R and Perl scripts | https://github.com/yupchen/sizer-paper | N/A |
| Bowtie2 | http://bowtie-bio.sourceforge.net/bowtie2/index.shtml | N/A |
| SeqMonk | https://www.bioinformatics.babraham.ac.uk/projects/seqmonk/ | N/A |