Antibodies |
HA Tag recombinant rabbit polyclonal |
Invitrogen |
8HCLC |
TAP Tag rabbit polyclonal |
Invitrogen |
CAB1001 |
Anti-Arp7 goat polyclonal |
Santa Cruz |
Sc-8961 |
Donkey anti-mouse |
LI-COR |
IRDye 800Cw |
Donkey anti-rabbit |
LI-COR |
IRDye 800Cw |
Donkey anti-goat |
LI-COR |
680RD |
Chemicals, Peptides, and Recombinant Proteins |
1NMPP1 (4-Amino-1-tert-butyl-3-(1’-naphthylmethyl)pyrazolo[3,4-d]pyrimidine) |
Toronto Research Chemicals |
A603003 |
Low Fluorescence Yeast Nitrogen Base |
US Biological Life Sciences |
Y2025–01 |
Yeast Nitrogen Base w/o AA, carb & w/AS |
US Biological Life Sciences |
Y2025 |
Yeast Extract |
US Biological Life Sciences |
Y2010 |
Peptone-Y |
Bio101 |
4018–532 |
DMSO (dimethylsulfoxide) |
J.T. Baker |
9224–01 |
cOmplete protease inhibitor cocktail |
Millipore-Sigma |
11697498001 |
Bradford dye reagent |
BioRad |
5000006 |
Laemmli protein sample buffer |
BioRad |
1610747 |
Precision Plus Protein Dual Color standards |
BioRad |
1610374 |
HA Tag Recombinant rabbit polyclonal antibody |
Invitrogen |
8HCLC |
Sytox green |
Thermo Fisher |
S7020 |
Critical Commercial Assays |
Ovation Universal RNA-Seq System, Custom |
NuGen |
N/A |
Deposited Data |
BAM files from RNA-Seq |
GEO |
GSE145206 |
Experimental Models: Organisms/Strains |
Yeast Deletion Set, strains BY4741, BY4742 |
Horizon (current source) |
N/A |
YC31–1c MATα leu2 ura3
|
This work |
N/A |
YC34–1a cln2::CLN2prWHI5 whi5::WHI5prCLN2
|
This work |
N/A |
YC18–2a MATα cdc28-as
|
This work |
N/A |
YC31–2a MATα leu2 ura3 cln2::kanMX whi5::kanMX
|
This work |
N/A |
YCT01 MATα leu2 ura3 whi5::kanMX
|
This work |
N/A |
YCT02 MATa leu2 ura3 cln2::kanMX
|
This work |
N/A |
YCT03a MATα leu2 ura3 whi5::WHI5pr-CLN2
|
This work |
N/A |
YC32–2d MATα leu2 ura3 cln2::kanMX whi5::WHI5pr-CLN2
|
This work |
N/A |
YC33–1d MATα leu2 ura3 cln2::CLN2pr-WHI5 whi5::kanMX
|
This work |
N/A |
YCT04 MATα leu2 ura3 cln2::CLN2pr-WHI5
|
This work |
N/A |
GZ238 MATa leu2 ura3
|
Zhao et al. 2016 |
N/A |
GZ240 MATa
|
Zhao et al. 2016 |
N/A |
GZ241 MATalpha
|
Zhao et al. 2016 |
N/A |
BF532–2a cdc28-as 5xGFP11-CLN3 gal1,10::TEF-GFP1–10 mCherry-PEST::LEU2 ade2 his3 ura3 leu2
|
This work |
N/A |
BF530–6b cdc28-as SIC1-TAP
|
This work |
N/A |
BF530–6b cdc28-as WHI2-TAP
|
This work |
N/A |
CLN2C cdc28-as |
This work |
N/A |
WHI3-GFP (BY4741 background) |
Cai and Futcher 2013 |
N/A |
Recombinant DNA |
pRS425_TEF1_GFP1–10_IDT |
This work |
N/A |
Pap_6GFP_HIS5 |
This work |
N/A |
pRS426_GAL1_sc5xGFP11_CLN3 |
This work |
N/A |
Software and Algorithms |
R Programming language |
http://www.rproject.org |
N/A |
Custom R and Perl scripts |
https://github.com/yupchen/sizer-paper |
N/A |
Bowtie2 |
http://bowtie-bio.sourceforge.net/bowtie2/index.shtml |
N/A |
SeqMonk |
https://www.bioinformatics.babraham.ac.uk/projects/seqmonk/ |
N/A |