Figure 2.

To generate Ezh2cKO, mice in which both copies of the Ezh2 gene were floxed (Ezh2^flox/flox) were crossed with mice expressing cre recombinase driven by the progesterone receptor promoter Pgr^wt/Cre. The first cross incorporates the cre recombinase that generates an EZH2 deletion in one of the two alleles. Offspring of this cross were mated to obtain males expressing (Pgr^wt/Cre, Ezh2^flox/flox). These males were then mated with Pgr^wt/wt, Ezh2^flox/flox females. Half of the females produced by this cross are a Pgr^wt/Cre, Ezh2^flox/flox expressing cre recombinase and with both EZH2 alleles floxed (Ezh2cKO). The other half are Pgr^wt/wt, Ezh2^flox/flox WT controls.