Fig 4. CD74^−/− HSPCs show a higher potential to repopulate the BM.

Lethally irradiated WT CD45.1 recipient mice were reconstituted with 7.5*10⁴ sorted LSK cells from WT (CD45.1), and 7.5 × 10⁴ sorted LSK from CD74^−/− (CD45.2) at a 1:1 ratio. Percent of donor-derived cells was analyzed in the BM after 6 and 18 weeks. (A) Total BM cells; Data A in S4 Data (B) myeloid cells; Data B in S4 Data (C) immature BM B cells; Data C in S4 Data and (D) mature BM B cells, Data D in S4 Data. (E) Percent of donor-derived cells was analyzed in LSK and CD34-LSK cells 18 weeks posttransplant. n = 6–8. Data E in S4 Data. n = 6–8. Bars show SEM. Unpaired two-tailed t test *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. BM, bone marrow; HSPC, hematopoietic stem and progenitor cell; WT, wild-type.