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. 2021 Mar 16;10:e64250. doi: 10.7554/eLife.64250

Figure 1. Identification of putative mechanosensory channels in the Venus flytrap trigger hair.

(A) Representative image of a soil-grown Venus flytrap clone (left), Venus flytrap leaf (center), and single trigger hair (right). Black arrowheads in the center picture indicate trigger hairs on leaf. (B) Scanning electron micrograph of a trigger hair. Cells of the lever (L), indentation zone (In) and podium (P) are indicated. Also seen are the digestive glands on the floor of the lobe. (C) Fold enrichment of protein-coding genes of >100 amino acids in length (black circles) in the trigger hair relative to the trap. FLYC1, FLYC2, and OSCA are shown in red, orange, and green, respectively. CPM, counts per million of mapped sequencing reads. (D) Average Fragments Per Kilobase of transcript per Million mapped reads (FPKM) for FLYC1, FLYC2, and OSCA in traps and trigger hairs. Dots of the same color indicate paired biological replicates. **FDR < 0.005.

Figure 1—source data 1. Size estimates of two Arabidopsis (Col-0) samples compared to our Venus flytrap strain (CP01).
Figure 1—source data 2. Summary of sequencing reads used to build the de novo transcriptome (NCBI Transcriptome Shotgun Assembly Sequence Database accession # GHJF00000000).

Figure 1.

Figure 1—figure supplement 1. Venus flytrap clonal propagation system.

Figure 1—figure supplement 1.

(A) Example of clonal Venus flytraps growing in tissue culture (10 cm plate) using methods adapted from Jang et al., 2003). (B) Diagram depicting the method of propagation. Rosettes were separated by splitting the rhizome. Plants were then transferred to fresh sterile growth medium for further propagation in culture or transferred to soil and ‘hardened’ for at least 2–3 months prior to experiments.

Figure 1—figure supplement 2. Gene structure of Venus flytrap FLYC1.

Figure 1—figure supplement 2.

A comparison of Arabidopsis MSL10 and Venus flytrap FLYC1 genes. Boxes represent exons; lines, introns. The sequence between the start and stop codons only is shown. SNPs (allelic differences) in FLYC1 are indicated for our strain (CP01) by black arrowheads. Thirty-two SNPs in total were detected, of which only two were found in the coding region and were both silent. Additional SNPs and short sequence variations found in the cultivar 'Creeping Death' are indicated by blue arrowheads (silent variations) and red (causing amino acid changes P8S and A51G relative to strain CP01).

Figure 1—figure supplement 3. Gene sequence of FLYC1.

Figure 1—figure supplement 3.

Exons, uppercase and bold; introns, lower case. SNPS and sequence variations identified in the strain CP01 and "Creeping Death" cultivar are shown in square brackets and are highlighted in red.