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Whole mounts of fetal cerebrum at (A) 18 and (B) 25 gestational weeks stained with Alcian blue to demonstrate acid mucopolysaccharide, which forms most extracellular matrix in the developing brain. At 18 gestational weeks, acid mucopolysaccharide (blue) is abundant in the subplate layer but absent in the intermediate zone. By 25 gestational weeks, acid mucopolysaccharide is present in the intermediate zone.
