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. Author manuscript; available in PMC: 2021 Mar 17.
Published in final edited form as: Bioconjug Chem. 2019 Nov 26;30(12):3098–3106. doi: 10.1021/acs.bioconjchem.9b00755

Figure 2.

Figure 2.

(a) The stability of conjugate 1 in aqueous solution, cell culture media, and human serum at 37 °C. Each experiment was repeated twice and error bars show standard deviation. (b) Analysis of different moieties present in solution during incubation of conjugate 1 with human serum using RP-HPLC. (c) Structures of the possible molecules/adducts when conjugate 1 is incubated in human serum (due to the reversible thiol-maleimide reaction) or in acidic conditions. The numbers in parenthesis are the calculated mass [M+H]+.