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. Author manuscript; available in PMC: 2021 Oct 1.
Published in final edited form as: Nat Immunol. 2020 Aug 24;21(10):1205–1218. doi: 10.1038/s41590-020-0758-6

Fig. 2 |. Nkg7 expression is enriched in NK cells at steady state and inducible in CD4+ T cells.

Fig. 2 |

a, A mouse expressing the Cre gene behind the promoter of Nkg7 was generated (B6.Nkg7-cre) and crossed to a membrane reporter (B6.mT/mG) to generate Nkg7 reporter mice (Nkg7-cre × mT/mG). Validation of GFP expression was performed on splenic NK cells in naive Cre+ mice. b, t-SNE plot of splenocytes from a naive mouse, pre-gated to exclude doublets, dead cells and NK1.1-APC/Cy7−TCR-β-BUV737− cells. The remaining cells were clustered using NK1.1-APC/Cy7, TCR-β-BUV737, CD4-BUV395 and CD8α-PE/Cy7. Equal numbers of cells (50,000 cells) are shown for Cre− and Cre+ plots. n = 1 per genotype, performed once. c, The expression of Nkg7 (GFP+) under TH0 (anti-CD3 + anti-CD28 + recombinant IL-2), TH1 (recombinant IL-12 + anti-IL-4 + TH0 conditions), TR1 (recombinant IL-27 + TH0 conditions), Treg (recombinant IL-27 + recombinant TGF-β + TH0 conditions), TH2 (recombinant IL-4 + anti-IFN-γ + TH0 conditions) and TH17 (recombinant IL-6 + recombinant IL-1β + recombinant IL-23 + anti-IFN-γ + anti-IL-4 + TH0 conditions) cell polarizing conditions. n = 1 per genotype. Plots are representative of two independent experiments. d, Expression of Nkg7 (GFP+) when recombinant IL-27 was titrated, or when recombinant TGF-β was titrated in the presence of recombinant IL-27. n = 1 per genotype, performed once. TH, T helper cells; TR1, type 1 regulatory cells; Treg cells, inducible regulatory T cells. See also Extended Data Fig. 1.