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. 2021 Mar 3;8:570982. doi: 10.3389/fvets.2021.570982

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Copyright © 2021 Quinlan, May, Weeks, Yuan and Luff.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Upregulation of p53 and p53-regulated genes p21 and Bax in canine keratinocytes in response to exposure to ultraviolet B (UVB) light. Normal canine keratinocytes were exposed to UVB (10 mJ/cm²) and cell lysates collected at different times (hours) post-irradiation. Proteins were separated by SDS-PAGE and analyzed by immunoblotting with antibodies directed against p53, p21, and Bax. β-actin was used a loading control. One representative experiment of three is shown.