Figure 2.

Upregulation of long non-coding (lnc.) RNA-p21 in canine keratinocytes in response to exposure to ultraviolet B (UVB) light. Normal canine keratinocytes were exposed to UVB (10 mJ/cm²) and cell lysates collected at different times points post-irradiation for RNA isolation. Real time PCR was performed to determine expression of lncRNA-p21. Resulting Cq values were normalized to the Cq value of the reference gene and calibrated to transcript levels in non-UVB exposed keratinocytes. Experiments were performed in duplicate and repeated in three independent experiments. Results of one representative experiment is shown and expressed as mean ± SD. **p < 0.01; ***p < 0.001; ****p < 0.0001.