Figure 3.

Upregulation of p53 and p53-regulated genes in UVB-exposed canine keratinocytes expressing CPV2 E6. Canine keratinocytes expressing either vector only or CPV2 E6 were exposed to UVB (10 mJ/cm²) and cell lysates collected at 12 and 24 h post-irradiation. (A) Proteins were separated by SDS-PAGE and analyzed by immunoblotting with antibodies directed against p53, p21, Bak, and Bax. β-actin was used a loading control. One representative experiment of three is shown. (B) Real time PCR was performed to determine expression of lncRNA-p21. Resulting Cq values were normalized to the Cq value of the reference gene and calibrated to transcript levels in vector only non-UVB exposed cells. Experiments were performed in duplicate and repeated in three independent experiments. One representative experiment of three is shown. Results are expressed as mean ± SD. *p < 0.05.