TABLE 2.
Candidate genes associated with pod number.
| Pod number |
High |
Low |
||||||||
| Published QTL marker | Ref. | SNP id | NR | Ref | NR | Ref | p-value | Candidate gene | Fold change | Gene function |
| Chr11:15649090 | 1 | ss715609881 | 11 | 1# | 0 | 12$ | 9.60E-06 | Glyma.11G164800 | 3.17* | LOB domain-containing protein 4 |
| Chr15:5252046 | 1 | ss715622826-7 | 3 | 9 | 11 | 1 | 2.80E-03 | Glyma.15G068500 | 8.6* | 60S ribosomal protein L26-1-like |
| Chr18:55626229 | 2 | ss715632229 | 5 | 7 | 12 | 0 | 4.60E-03 | Glyma.18G274000 | 16.83* | RING finger protein 165-like |
| Chr11:5338661 | 3 | ss715610851-6 | 8 | 4 | 1 | 11 | 9.40E-03 | Glyma.11G071000 | 1.28* | Auxilin-like protein 1-like |
| Chr02:40368201 | 3 | ss715582578 | 6 | 6 | 12 | 0 | 1.40E-02 | Glyma.02G216500 | 2.44* | TRAF-type zinc finger-related |
| Chr02:40368201 | 3 | ss715582578 | 6 | 6 | 12 | 0 | 1.40E-02 | Glyma.02G216600 | 1.39* | AGAMOUS-like 16 |
| Chr11:33902439 | 1 | ss715610584 | 8 | 4 | 2 | 10 | 3.60E-02 | Glyma.11G245300 | 0.72 | 63 kDa inner membrane protein |
| Chr18:55662445 | 2 | ss715632230 | 7 | 5 | 12 | 0 | 3.70E-02 | Glyma.18G274200 | 0.08 | Pollen Ole e1 extensin family protein |
NR, non-reference allele; Ref, reference allele. The numbers of NR and Ref indicate the number of varieties out of 24 PIs. Low and High are the pod numbers and are defined as pod numbers below or above average, respectively. The p-values were calculated using fisher’s exact test. Candidate genes were selected with 50 Kb window of enriched SNP based on published 50K SNP data. References: 1. Zhang J. et al. (2015). 2. Fang et al. (2017). 3. Hao et al. (2012). SNP id ss715622826-7 means ss715622826 and ss715622827. SNP id ss715610851-6 means ss715610851, ss715610852, ss715610853, ss715610854, ss715610855, and ss715610856. The genes marked with asterisk (*) have log2fold change > 1 indicating the candidate genes are expressed more than two-fold higher in shoot apical meristem than in leaves. # and $ mark the numbers described in the main text.