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. 2021 Mar 3;12:629045. doi: 10.3389/fpls.2021.629045

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Copyright © 2021 Chabi, Leleu, Fermont, Colpaert, Colleoni, Ball and Cenci.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Phylogenetic analysis of glycogen phosphorylase (GT35 family). The tree displayed is midpoint rooted and represents the consensus tree obtained with Phylobayes 4.1 with ML bootstrap values drawn from 100 bootstraps repetition with IQTREE (left) and Bayesian posterior probabilities (right) mapped onto the nodes. Bootstrap values >50% are shown, while only posterior probabilities >0.6 are shown. The scale bar shows the inferred number of amino acid substitutions per site. Sequences are highlighted in purple for Stramenopila, brown for Bacteria, while everything else is in black. Sequence names are composed of the organism name, the accession number, and their clades. We can see that Bicosoecida, Halocafeteria, and Cafeteria group together, with a high posterior probability (pp = 1). However, Blastocystis was not found inside a monophyletic group with Stramenopila but was found among other eukaryotes. The Blastocystis position is probably due to the fast-evolving sequence in this organism as it has been observed in several studies (Eme et al., 2017; Moreira and López-García, 2017).