Figure 1.

Effect of nisin on milk acidification profiles and phenotypic classification of 710 L. lactis strains. Milk acidification curves of strains grown at 30°C without nisin (CFS_control) and with 1.5 μg ml⁻¹ nisin addition (CFS_nisin) The thin lines depict the averaged milk acidification data of three replicates while the thicker line depicts the averaged curves per group. The nisin-containing milk phenotypes are classified to four groups; (A) LA, loss of acidification; (B) HDA, highly delayed acidification; (C) MDA, mildly delayed acidification; and (D) UA, unaltered acidification. All acidification curves are synchronized based on acidification onset of each respective CFS_control profile and normalized to a start pH of 6.4.