Fig. 2.

Proliferative properties of bone-related cell cultures grown on BCM-coated DBBM. a, b Proliferative ability of ST2, MC3T3-E1, and primary bone–derived cells grown on DBBM coated with BCM-RS (a) or BCM-RS + S (b), was assessed by BrdU incorporation into newly synthesized DNA immediately after plating (0 h) as well as at 24, 48, 72, and 96 h. Cells of each cell type grown on BCM-free DBBM that is hydrated with RS (a) or RS + S (b) are used as controls (Ctrl). Experimental values were normalized to the values of control cells at the time point 0. Means ± SD from three independent experiments and significant differences to control cells unless otherwise indicated, ***P < 0.001, **P < 0.01, *P < 0.05 are shown