Fig. 5.

BCM-coated DBBM induces Alpl gene expression in bone-related cell cultures. a, b ST2, MC3T3-E1, and primary bone-derived cells were grown on DBBM coated with BCM-RS (a) or BCM-RS + S (b) for 1, 3, and 7 days before total RNA was isolated and analyzed for the expression of the osteogenic marker gene Alpl by qRT-PCR. Controls (Ctrl) represent cells of each cell type seeded on BCM-free DBBM hydrated with RS (a) or RS + S (b). Values normalized to Gapdh are expressed relative to the values of control cells at the “1 day” time point. Please note the differences in the scales of the y-axis. Data represent means ± SD from three independent experiments. Significant differences to the respective controls at day 1 unless otherwise indicated, ***P < 0.001, **P < 0.01, *P < 0.05