Fig. 8.

Additive effect of fresh bone chips and BCM-coated DBBM on the Alpl and Bglap2 gene expression in bone-related cell cultures. a, b Effect of BCM-coated DBBM in the absence or presence of fresh bone chips on Alpl (a) and Bglap2 (b) mRNA levels in ST2, MC3T3-E1, and primary bone–derived cells. Cells were grown under each of the four experimental conditions described in Fig. 7a for 1, 3, and 7 days before total RNA was extracted and analyzed by qRT-PCR. Values normalized to Gapdh are expressed relative to the values of control cells at the time point “1 day.” Please note the differences in the scales of the y-axis. Data represent means ± SD from three independent experiments. Significant differences to the respective controls at day 1 unless otherwise indicated, ***P < 0.001, **P < 0.01, *P < 0.05