Fig. 3.

Timeline of the use of CRISPR/Cas9 for lineage tracing. a In situ mutagenesis involved the accumulation of multiple integration target sites (called barcoded scratchpads) visualized by smFISH. This strategy is a good tool to address the lineage dynamics of a cell population. b In vivo lineage mapping using CRISPR/Cas9 is based on multiple integration/deletion at a target site that allows the lineage trees of the different cells to be reconstructed taking into account their genomic tags (yellow cassettes). c Going further, the inducible Cas9 activity (blue circle) and the incorporation of transcriptomic analysis improves these mouse models, enabling cell lineages and fate mapping to be analyzed at the single-cell level