Fig. 3.

Factors affecting the distribution of peroxisomes in mammalian cells. Peroxisomes are distributed homogenously in several cell lines including Miro1/2 mouse embryonic fibroblasts. Depletion of members of the peroxisomal trafficking machinery (PEX14, KIFC3) causes a perinuclear positioning of peroxisomes, while changes in levels of other peroxisomal proteins lead to cytoplasmic clustering (e.g., overexpression of Miro1 variant 4, PEX1 or PEX5 deficiency, PEX3-induced pexophagy). In cultured neurons, peroxisomes are mainly found in the somata and proximal dendrites. Overexpression of ACBD5, however, leads to peroxisomal redistribution throughout the neurites and the periphery of somata. DKO, double knockout; OE, overexpression; KO, knockout; ↓, deficiency; var4, splice variant-4