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. 2021 Mar 12;14:1883–1893. doi: 10.2147/OTT.S286666

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© 2021 Deng et al.

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Overexpressing miR-34a inhibited CSCs-like properties, invasion and migration of PDAC.

Notes: Levels of miR-34a and HOTAIR expression in each group of CFPAC-1 (A and B) and SW-1990 cells (H and I) were detected by qRT-PCR. (C, D, J and K) The expression of CSCs markers, SOX2, Nanog and Oct4 were detected by Western bolt and qRT-PCR in each group, respectively. (E and L) CCK-8 assay was performed to detect the cell proliferation. (F and M) Sphere formation assay was performed to detect the sphere formation; spheres (>40 μm in diameter) were counted. (G and N) Transwell assay was performed to detect invasion and migration. Error bars: mean ± SD (n=3 for each group). *P<0.05; **P<0.01 ago-NC or antago-NC group.