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. 2021 Mar 16;12:1696. doi: 10.1038/s41467-021-21848-3

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© The Author(s) 2021, corrected publication 2021

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Fig. 4 — HPLC-MS analysis of cysteamine-unloaded and derivatized substrate from the CysH protein. EICs 411.1 m/z [M + H]⁺ are shown in black and EICs 427.1 m/z [M + H]⁺ (Di(ethylcarbonyl)-hydroxy-l-(iso)asparaginyl-dicysteamine) are shown in blue. a Di(ethylcarbonyl)-l-asparaginyl-dicysteamine synthetic reference. CysH-bound l-asparagine unloaded and derivatized showed the same retention time as the synthetic reference. Incubation of CysH with CysJ and l-asparagine lead to hydroxylation of l-asparagine and different retention times of the unloaded substrate compared to the reference. b Di(ethylcarbonyl)-l-isoasparaginyl-dicysteamine synthetic reference. CysH-bound l-isoasparagine unloaded and derivatized showed the same retention time as the synthetic reference. No hydroxylation occurred upon the addition of CysJ.