Fig. 3. Augmentation of IL-10 production by mannose core in vitro.

a–e Cytokine production by rpMϕ from WT(a–c) and Dectin-2KO (d, e) mice after stimulation with Man₉Core-BSA, Man₃Core-BSA, Man₅₁-BSA (bound more than 51 mannose monomers), and J-1012 N-glycan, immobilized on plates as in Fig. 2h. Schematic structures of Man₉Core-BSA and Man₃Core-BSA are depicted. f IL-10 production by BMDCs from WT and Dectin-2KO mice in response to Man₉Core-BSA. g IL-10 production by BMDCs from WT mice upon stimulation with Man₉Core-BSA, Man₃Core-BSA, Man₅₁-BSA, J-1012 N-glycan, and J-1012 N-glycan treated with α-mannosidase. h Schematic structure of (Man₉CoreCys)₅-biotin. εAHX, ε-amino-n-hexanoic acid. i Cytokine production by BMDCs from WT and Dectin-2KO mice upon stimulation with (Man₉CoreCys)₅-biotin bound to an avidin-coated plate. These experiments were repeated at least twice, and representative results are shown. Data are expressed as the mean ± SD. p value was determined by unpaired two-tailed Student’s t-test. N.S., not significant (p > 0.05).