Fig. 1.

GFP expression in SLK-BAC16-mCherryORF45-infected cells following targeting of selected genes by CRISPR-Cas9. BAC16-mCherryORF45-infected SLK cells expressing Cas9 were transduced with recombinant lentiviruses encoding a random non-targeting sgRNA (control sgRNA) or sgRNAs targeting gfp, orf45 and orf73. Each gene was targeted with a combination of two guides. Transduced cells were selected with 1 µg/ml puromycin and were grown in the absence or presence of 600 µg/ml hygromycin. Cells were harvested 6, 9, 13 and 16 days post transduction, and GFP expression was monitored by FACS analysis. Mock treated SLK-BAC16 cells that were either maintained in the presence or absence of hygromycin were used as controls. *p < 0.05, **p < 0.01, ***p < 0.001. Statistical test was performed between groups including all time points together