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. 2021 Mar 17;18:56. doi: 10.1186/s12985-021-01527-x

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — Sequencing the episome CRISPR-Cas9 cut sites. To map insertions and deletions (indels), we amplified genomic DNA and subjected it to deep sequencing. The percentage on the y axis represents the total number of reads with indels divided by the total numbers of reads. The identities of the samples are indicated on the x axis, including the name of the gene and the number of days post sgRNA transduction. Percentage of indels flanking the cutting site in gfp using the hygromycin minus sgGFP samples (a). Percentage of indels flanking the cutting site in orf73 using both hygromycin minus and hygromycin plus samples (b). Percentage of indels around the cutting site in orf45 using hygromycin minus samples (c)