Table 2.

Primers used for PCR amplification and sequencing. Column 1: Gene name. Column 2: primers sequence. Column 3: PCR product length

Gene	Primer pair	*Size of PCR product
gfp	1st F: TCTTCTTCAAGGACGACGGC 2nd F: GCAGCTCGCCGACCACTAC 1st + 2nd R: TTACTTGTACAGCTCGTCCA	462 bp 226 bp
orf73	1st F: AGATGTGACCTTGGCGATG 2nd F: CAACCGCGAAGGAAGCAT 1st + 2nd R: CGGAGACACAGGATGGGATG	313 bp 282 bp

^*Sizes ignore potential deletions and insertions due to single or double cut by Cas9