Table 2.

Primers used for virological analysis

Agent	Target gene	Amplicon (size bp)	References
CDV	Nucleoprotein	287	[21]
CAdV-1	E3	508	[22]
CAdV-2	E3	1030	[22]
CaHV-1	Glycoprotein B	450	[23]
CPV-2	VP2 protein	583	[8, 24]

CDV canine distemper virus, CAdV-1 canine adenovirus type 1, CAdV-2 canine adenovirus type 2, CaHV-1 canid alphaherpesvirus 1, CPV-2 canine parvovirus 2, bp base pairs