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. 2020 Nov 25;52(1):467–475. doi: 10.1007/s42770-020-00410-1

Table 2.

Summary of results from 30 bovine serum samples positive for BVDV by virus isolation, antigen-capture ELISA, nested RT-PCR, and real-time RT-PCR

Diagnostic tests
Serum VIa AgELISAb RT-nPCRc RT-qPCRd Clinical status
Result Biotype Result Species Result Cq
1 + ncp + + 1 + 26.9 PI
2 + ncp + + 1 + 25.3 PI
3 + ncp + + 1 + 29.6 PI
4 + ncp + + 1 + 27.5 PI
5 + ncp + + 1 + 26.4 PI
6 + ncp + + 1 + 31.8 necropsye
7 + ncp + + 1 + 26.7 PI
8 + ncp + + 1 + 26.4 PI
9 + ncp + + 1 + 28.8 PI
10 + ncp + + 1 + 26.1 PI
11 + ncp + + 1 + 23.4 unknownf
12 + ncp + + 1 + 21.4 unknown
13 + ncp + + 1 + 24.2 necropsy
14 + ncp + + 1 + 25.9 PI
15 + ncp + + 1 + 26.4 PI
16 + ncp + + 1 + 27.7 PI
17 + ncp + + 1 + 24.6 PI
18 + ncp + + 1 + 29.6 PI
19 + ncp + + 1 + 29.3 PI
20 + ncp + + 1 + 27.9 PI
21 + ncp + + 1 + 29.1 PI
22 + ncp + + 1 + 23.2 PI
23 + ncp + 1 + 25.7 unknown
24 - NA + + 1 + 24.0 unknown
25 - NA + 1 + 33.4 unknown
26 - NA + + 1 + 25.9 PI
27 - NA + NA NA unknown
28 - NA + NA NA unknown
29 - NA + NA NA unknown
30 - NA + NA + 34.6 unknown

aVirus isolation (Odeón et al., 2003) [14]

bAntigen-capture ELISA, INGEZIM BVD DAS, Ingenasa, Spain

cNested RT-PCR (Gilbert et al., 1999) [16]

dReal-time RT-PCR (Mari et al., 2016) [19]

eAnimal with clinicopathological findings compatible with bovine viral diarrhea-mucosal disease

fNot retested to confirm PI status

ncp, non-cytopathic; Cq, quantification cycle value; PI, persistent infected animal; NA, not applicable