Table 2.
Summary of results from 30 bovine serum samples positive for BVDV by virus isolation, antigen-capture ELISA, nested RT-PCR, and real-time RT-PCR
| Diagnostic tests | ||||||||
|---|---|---|---|---|---|---|---|---|
| Serum | VIa | AgELISAb | RT-nPCRc | RT-qPCRd | Clinical status | |||
| Result | Biotype | Result | Species | Result | Cq | |||
| 1 | + | ncp | + | + | 1 | + | 26.9 | PI |
| 2 | + | ncp | + | + | 1 | + | 25.3 | PI |
| 3 | + | ncp | + | + | 1 | + | 29.6 | PI |
| 4 | + | ncp | + | + | 1 | + | 27.5 | PI |
| 5 | + | ncp | + | + | 1 | + | 26.4 | PI |
| 6 | + | ncp | + | + | 1 | + | 31.8 | necropsye |
| 7 | + | ncp | + | + | 1 | + | 26.7 | PI |
| 8 | + | ncp | + | + | 1 | + | 26.4 | PI |
| 9 | + | ncp | + | + | 1 | + | 28.8 | PI |
| 10 | + | ncp | + | + | 1 | + | 26.1 | PI |
| 11 | + | ncp | + | + | 1 | + | 23.4 | unknownf |
| 12 | + | ncp | + | + | 1 | + | 21.4 | unknown |
| 13 | + | ncp | + | + | 1 | + | 24.2 | necropsy |
| 14 | + | ncp | + | + | 1 | + | 25.9 | PI |
| 15 | + | ncp | + | + | 1 | + | 26.4 | PI |
| 16 | + | ncp | + | + | 1 | + | 27.7 | PI |
| 17 | + | ncp | + | + | 1 | + | 24.6 | PI |
| 18 | + | ncp | + | + | 1 | + | 29.6 | PI |
| 19 | + | ncp | + | + | 1 | + | 29.3 | PI |
| 20 | + | ncp | + | + | 1 | + | 27.9 | PI |
| 21 | + | ncp | + | + | 1 | + | 29.1 | PI |
| 22 | + | ncp | + | + | 1 | + | 23.2 | PI |
| 23 | + | ncp | – | + | 1 | + | 25.7 | unknown |
| 24 | - | NA | + | + | 1 | + | 24.0 | unknown |
| 25 | - | NA | – | + | 1 | + | 33.4 | unknown |
| 26 | - | NA | + | + | 1 | + | 25.9 | PI |
| 27 | - | NA | + | – | NA | – | NA | unknown |
| 28 | - | NA | + | – | NA | – | NA | unknown |
| 29 | - | NA | + | – | NA | – | NA | unknown |
| 30 | - | NA | + | – | NA | + | 34.6 | unknown |
aVirus isolation (Odeón et al., 2003) [14]
bAntigen-capture ELISA, INGEZIM BVD DAS, Ingenasa, Spain
cNested RT-PCR (Gilbert et al., 1999) [16]
dReal-time RT-PCR (Mari et al., 2016) [19]
eAnimal with clinicopathological findings compatible with bovine viral diarrhea-mucosal disease
fNot retested to confirm PI status
ncp, non-cytopathic; Cq, quantification cycle value; PI, persistent infected animal; NA, not applicable