Table 2.
Diagnostic tests | ||||||||
---|---|---|---|---|---|---|---|---|
Serum | VIa | AgELISAb | RT-nPCRc | RT-qPCRd | Clinical status | |||
Result | Biotype | Result | Species | Result | Cq | |||
1 | + | ncp | + | + | 1 | + | 26.9 | PI |
2 | + | ncp | + | + | 1 | + | 25.3 | PI |
3 | + | ncp | + | + | 1 | + | 29.6 | PI |
4 | + | ncp | + | + | 1 | + | 27.5 | PI |
5 | + | ncp | + | + | 1 | + | 26.4 | PI |
6 | + | ncp | + | + | 1 | + | 31.8 | necropsye |
7 | + | ncp | + | + | 1 | + | 26.7 | PI |
8 | + | ncp | + | + | 1 | + | 26.4 | PI |
9 | + | ncp | + | + | 1 | + | 28.8 | PI |
10 | + | ncp | + | + | 1 | + | 26.1 | PI |
11 | + | ncp | + | + | 1 | + | 23.4 | unknownf |
12 | + | ncp | + | + | 1 | + | 21.4 | unknown |
13 | + | ncp | + | + | 1 | + | 24.2 | necropsy |
14 | + | ncp | + | + | 1 | + | 25.9 | PI |
15 | + | ncp | + | + | 1 | + | 26.4 | PI |
16 | + | ncp | + | + | 1 | + | 27.7 | PI |
17 | + | ncp | + | + | 1 | + | 24.6 | PI |
18 | + | ncp | + | + | 1 | + | 29.6 | PI |
19 | + | ncp | + | + | 1 | + | 29.3 | PI |
20 | + | ncp | + | + | 1 | + | 27.9 | PI |
21 | + | ncp | + | + | 1 | + | 29.1 | PI |
22 | + | ncp | + | + | 1 | + | 23.2 | PI |
23 | + | ncp | – | + | 1 | + | 25.7 | unknown |
24 | - | NA | + | + | 1 | + | 24.0 | unknown |
25 | - | NA | – | + | 1 | + | 33.4 | unknown |
26 | - | NA | + | + | 1 | + | 25.9 | PI |
27 | - | NA | + | – | NA | – | NA | unknown |
28 | - | NA | + | – | NA | – | NA | unknown |
29 | - | NA | + | – | NA | – | NA | unknown |
30 | - | NA | + | – | NA | + | 34.6 | unknown |
aVirus isolation (Odeón et al., 2003) [14]
bAntigen-capture ELISA, INGEZIM BVD DAS, Ingenasa, Spain
cNested RT-PCR (Gilbert et al., 1999) [16]
dReal-time RT-PCR (Mari et al., 2016) [19]
eAnimal with clinicopathological findings compatible with bovine viral diarrhea-mucosal disease
fNot retested to confirm PI status
ncp, non-cytopathic; Cq, quantification cycle value; PI, persistent infected animal; NA, not applicable