Table 3.

Agreement among virus isolation, antigen-capture ELISA, nested RT-PCR, and real-time RT-PCR for BVDV detection in 90 bovine serum samples

	Percentage agreemente		Gwet’s Agreement Coefficientf
Diagnostic tests	Negative	Positive	AC1	CI95%	Level of agreementg
VIa vs. AgELISAb	94.6	86.3	0.87	0.77–0.98	Moderate to almost perfect
VI vs. RT-nPCRc	97.7	93.9	0.94	0.88–1.00	Strong to almost perfect
VI vs. RT-qPCRd	96.2	89.8	0.93	0.85–1.00	Strong to almost perfect
AgELISA vs. RT-nPCR	95.2	88.9	0.88	0.79–0.98	Moderate to almost perfect
AgELISA vs. RT-qPCR	96.0	90.9	0.90	0.82–0.99	Strong to almost perfect
RT-nPCR vs. RT-qPCR	99.2	98.1	0.98	0.94–1.00	Strong to almost perfect

^aVirus isolation (Odeón et al., 2003) [14]

^bAntigen-capture ELISA, INGEZIM BVD DAS, Ingenasa, Spain

^cNested RT-PCR (Gilbert et al., 1999) [16]

^dReal-time RT-PCR (Mari et al., 2016) [19]

^eEstimated according to Cicchetti and Feinstein (1990) [20]

^fEstimated according to Gwet (2008) [21]

^gInterpretation according to McHugh (2012) [22]