Figure 7.

T09 cells have an overlapping Th17-Tfh phenotype characterized by targetable surface markers

(A) Gene set enrichment of T helper cell signatures derived from publicly available data¹⁸ projected on umap plots of the CD4 memory T cell (CD4mem) compartment. The localization of T09 cells in CD4mem is indicated in the lower right panel. All cohorts (MS1–3 and HI1–3) are shown.

(B) Differential gene expression (DEG) analysis contrasting single-cell transcriptomes of T09 cells (n = 19,874) and other CD4mem cells (n = 73,002). All cohorts (MS1–3 and HI1–3) were used.

(C) Mean mRNA expression per individual (n = 62; cohorts MS1–3 and HI1–3) for KLRB1 and LTB in CD4mem clusters.

(D) (Co-)expression of KLRB1 and LTB projected on umap for all cohorts (MS1–3 and HI1–3).

(E) Mean mRNA expression per individual (n = 62; cohorts MS1–3 and HI1–3) for selected T09 markers in CD4mem clusters.

(F) Top 100 uniquely enriched or de-enriched GO terms in T09 markers from (B). Nodes indicate GO terms; edges represent shared genes between GO terms.

(G) Schematic indicating the surface marker profile of T09 cells homing to the CNS of RRMS and progressive MS patients.