Table 3.

Status of HPV-16 sublineages isolated from different anatomical sites of HNSCC patients and immunoreactivity of p16INK4a and NF-κB biomarkers

No.	HPV16* lineage	HPV16 sublineage	Anatomic site	Primary tumor grade	p16INK4a expression**	Cytoplasmic NF-κB expression***	Nuclear NF-κB expressionC
1	A	A1	Oral cavity	Well differentiated	2+	High	Low
2	A	A1	Larynx	Moderately differentiated	3+	High	Low
3	A	A1	Larynx	Moderately differentiated	3+	High	High
4	A	A1	Oral cavity	Well differentiated	2+	High	Low
5	A	A2	Oral cavity	Well differentiated	3+	High	Low
6	A	A2	Larynx	Well differentiated	2+	High	High
7	A	A2	Hypopharynx	Well differentiated	3+	Low	Low
8	A	A2	Tonsil	Well differentiated	3+	High	Low
9	A	A2	Base of the tongue	Poorly differentiated	3+	High	High
10	A	A2	Base of the tongue	Poorly differentiated	3+	High	High
11	A	A2	Hypopharynx	Poorly differentiated	3+	High	Low
12	D	D2	Oral cavity	Well differentiated	2+	High	Low
13	D	D2	Base of the tongue	Well differentiated	3+	High	Low

*HPV, human papillomavirus. ** 4-Tier scale from 0 to 3+ for p16INK4a immunostaining according to Duncan et al. [22]. ***Low expression < 3 and high expression ≥ 3 for NF-κB immunostaining according to Zhou et al. [23]