Fig. 1.

In vitro sensitivity and resistance of Sporothrix brasiliensis isolates from feline cases in Pelotas/RS, Southern Brazil, to different itraconazole formulations. In 96-well microplates, the visualization of fungal growth is noted into wells containing the itraconazole concentrations ranging from 16 to 0.03 μg/mL, in comparison with the positive and negative controls, by the antifungal susceptibility assay (M38-A2 of the Clinical Laboratory Standard Institute). The fungistatic activity of a compounded formulation for human use, identified as H-COMP2 was observed in both duplicate test lines against a feline isolate, showing a MIC of 1 μg/mL (a). No fungistatic activity was observed in a brand-name formulation for veterinary use, identified as V-IND, which MIC was greater than 16 μg/mL (b) against another feline isolate