TABLE 3.
Polyhydroxyalkanoate accumulation in Ralstonia eutropha strain 005dC1Z126TRCB by expressing PhaCAc NSDG with S389X mutation.
| Expressed PhaCAc | Dry cell wt. (g/L) | PHA content (wt.%) | PHA concentration (g/L) | Residual cell mass (g/L) | 3HHx (mol%) | 3HO (mol%) | Size increment (Å3)a |
| NSDG | 17.8 ± 0.3 | 85.7 ± 0.8 | 15.2 ± 0.3 | 2.5 ± 0.1 | 13.1 ± 0.1 | trace | 0 |
| NSDG/S389A | 17.5 ± 0.3 | 84.3 ± 0.3 | 14.6 ± 0.0 | 2.7 ± 0.1 | 13.1 ± 0.1 | trace | −6 |
| NSDG/S389T | 16.9 ± 0.2 | 83.7 ± 1.4 | 14.1 ± 0.4 | 2.7 ± 0.2 | 14.9 ± 0.1 | 0.2 ± 0.1 | 20 |
| NSDG/S389V | 16.6 ± 0.3 | 84.2 ± 1.2 | 14.1 ± 0.5 | 2.8 ± 0.5 | 13.8 ± 0.2 | trace | 32 |
| NSDG/S389L | 17.8 ± 0.5 | 86.7 ± 1.1 | 15.4 ± 0.2 | 2.4 ± 0.4 | 13.5 ± 0.1 | trace | 51 |
| NSDG/S389I | 17.4 ± 0.3 | 78.7 ± 3.0 | 13.7 ± 0.1 | 3.7 ± 0.6 | 13.7 ± 0.1 | trace | 51 |
| NSDG/S389C | 17.6 ± 0.2 | 82.8 ± 0.3 | 14.6 ± 0.2 | 3.0 ± 0.1 | 13.7 ± 0.0 | trace | 13 |
Cells were cultured in mineral salt medium containing 1.5 w/v% palm kernel oil at 30 °C for 72 h.
Results are mean ± standard deviations from three separate experiments.
3HHx: 3-hydroxyhexanoate, 3HO: 3-hydroxyoctanoate, PHA: Polyhydroxyalkanoate, trace: less than 0.1 mol%.
aSize increment at position 389 from the wild-type PhaCAc.