TABLE 5.

Kinetic parameters of PHA granule-associated PhaC_Ac for R-3HB-CoA and R-3HHx-CoA substrates.

PhaCAc enzyme	R-3HB-CoA		R-3HHx-CoA
	Km (mM)a	kcat (min–1)b	Km (mM)a	kcat (min–1)b
Wild type	1.23	260	1.05	153
NSDG	1.26	599	0.73	259
NSDG/S389V	1.05	385	0.46	130
NSDG/S389T	1.34	1913	0.79	876
NSGD/S389C	1.30	898	0.53	304

^aMichaelis constant.

^bTurnover number calculated as one catalytic site in a dimerized PhaC_Ac.

PHA, Polyhydroxyalkanoate.