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. 2021 Mar 16;11:6037. doi: 10.1038/s41598-021-84050-x

Figure 1.

Figure 1

Flow chart of the infection assay. An exponential phase broth culture is prepared and used to inoculate microtiter plates containing confluent HUVEC layers using a multiplicity of infection of ∼ 16. After sedimentation of the bacteria by centrifugation at 300 g, the plates are incubated at 37 °C for 1½h. Then, 100 μg/ml gentamicin and 10 μg/ml lysostaphin is added to kill and lyse all extracellular bacteria. For sample collection at 1 hpi and 16 hpi, the plates are washed and trypsinized followed by snap freezing and storage at − 80 °C until RNA preparation.