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. 2021 Mar 16;11:5967. doi: 10.1038/s41598-021-85268-5

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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RFID internalization process procedure. (a) Drop an aliquot of the RFID solution into a cell well. (b) Once the isopropyl alcohol evaporates, the RFIDs remain at the well bottom. (c) Dispense a suspension of cells on top of the RFIDs. (d) After 24 h, many RFIDs are internalized inside the cells. In this microscope image of cultured cells, the arrows point to the RFIDs. (e) Dissociate the cells and RFID culture with Trypsin, as described in the methods section. (f) The result is a suspension of cells and cells with implanted RFIDs.