Table 2.
Predicted beta-lactam antimicrobial resistance genes and minimum inhibitory concentration of S. cohnii and S. urealyticus isolates SC1-15 from twelve German dairy farms.
| Strain | Species | Farm | Beta-lactam AMR1 genes | MIC2,3 (mg/L) | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| FOX | OXA | PEN | AMP | FEP | ETP | IMP | MERO | FOT | FOTCLA | TAZ | TAZCLA | TEMOCI | ||||
| SC1 | S. urealyticus | 1 | – | 8 | 0.5 | 0.5 | ≤ 1 | 4 | 2 | 0.25 | 0.5 | 8 | 8 | 32 | 32 | > 128 |
| SC2 | S. urealyticus | 2 | – | 8 | 0.5 | 0.25 | ≤ 1 | 4 | 2 | ≤ 0.12 | 0.5 | 8 | 8 | 32 | 32 | > 128 |
| SC3 | S. cohnii | 3 | – | 8 | 1 | 0.5 | ≤ 1 | 4 | 2 | ≤ 0.12 | 0.5 | 16 | 8 | 64 | 32 | > 128 |
| SC4 | S. urealyticus | 4 | – | 8 | 1 | 0.5 | ≤ 1 | 8 | 2 | ≤ 0.12 | 0.5 | 16 | 8 | 64 | 32 | > 128 |
| SC5 | S. cohnii | 5 | – | 8 | 1 | 0.5 | ≤ 1 | 4 | 2 | ≤ 0.12 | 0.5 | 8 | 8 | 64 | 32 | > 128 |
| SC6 | S. cohnii | 6 | – | 4 | 1 | 0.5 | ≤ 1 | 4 | 2 | ≤ 0.12 | 0.5 | 8 | 8 | 64 | 64 | > 128 |
| SC7 | S. cohnii | 7 | mecA | > 16 | > 8 | > 2 | 4 | > 32 | > 2 | 0.25 | 2 | > 64 | 64 | 128 | > 128 | > 128 |
| SC8 | S. cohnii | 7 | – | 8 | 0.5 | 0.5 | ≤ 1 | 4 | 2 | ≤ 0.12 | 0.5 | 8 | 8 | 32 | 32 | > 128 |
| SC9 | S. cohnii | 8 | – | 4 | 0.5 | 0.25 | ≤ 1 | 2 | 2 | ≤ 0.12 | 0.25 | 4 | 4 | 32 | 16 | > 128 |
| SC10 | S. cohnii | 8 | – | 8 | 0.5 | 0.5 | ≤ 1 | 4 | 2 | ≤ 0.12 | 0.5 | 8 | 8 | 32 | 32 | > 128 |
| SC11 | S. urealyticus | 9 | – | 4 | 0.5 | 0.25 | ≤ 1 | 2 | 2 | ≤ 0.12 | 0.5 | 8 | 4 | 64 | 32 | > 128 |
| SC12 | S. cohnii | 9 | – | 8 | 0.5 | 0.25 | ≤ 1 | 2 | 2 | ≤ 0.12 | 0.5 | 4 | 4 | 32 | 16 | > 128 |
| SC13 | S. cohnii | 10 | – | 4 | 0.5 | 0.25 | ≤ 1 | 2 | 2 | ≤ 0.12 | 0.5 | 8 | 8 | 32 | 32 | > 128 |
| SC14 | S. urealyticus | 11 | – | 4 | 0.5 | 0.25 | ≤ 1 | 2 | 2 | ≤ 0.12 | 0.5 | 4 | 4 | 16 | 16 | > 128 |
| SC15 | S. urealyticus | 12 | – | 8 | 0.5 | 0.5 | ≤ 1 | 2 | 2 | ≤ 0.12 | 0.5 | 8 | 4 | 32 | 16 | > 128 |
| EUCAST ECOFF5 | Staphylococcus aureus | ≤ 4 | ≤ 2 | ≤ 0.125 | ND | ≤ 8 | ND | ≤ 0.125 | ≤ 0.5 | ≤ 4 | ND | ND | ND | ND | ||
| Coagulase negative staphylococci | ND4 | ≤ 1 | ND | ND | ND | ND | ≤ 0.125 | ≤ 0.5 | ≤ 2 | ND | ND | ND | ND | |||
aAMR antimicrobial resistance, bMIC minimum inhibitory concentration, cFOX cefoxitin, OXA oxacillin, PEN penicillin, AMP ampicillin, FEP cefepim, ETP ertapenem, IMP imipenem, MERO meropenem, FOT cefotaxime, FOTCLA cefotaxime/clavulanic acid, TAZ ceftazidime, TAZCLA ceftazidime/clavulanic acid, TEMOCI temocillin; 4ND not defined. 5Epidemiological cut-off values as provided by EUCAST (http://www.eucast.org; accessed December 7, 2020).