Figure 1.

Experimental design and data acquisition. Bone marrow–derived macrophages (BMDMs) were harvested and cultured for 7 days either in the presence or absence of Se (as sodium selenite, Na₂SeO₃, 250 nM). BMDMs were stimulated with 100 ng of LPS for indicated time periods up to a maximum of 20 h. Cells were harvested after two washes with PBS and lysed to extract the proteome. Proteome was extracted and subjected to tryptic digestion followed by TMT labeling. Datasets were acquired on Lumos Fusion MS and analyzed by using Proteome Discoverer. Metabolites were extracted in methanol, dried, and reconstituted in the mobile phase with chlorpropamide as the internal standard. The dataset was acquired on Exactive Plus MS. Corresponding metabolite ion chromatograms were extracted using Xcalibur and inspected manually. LPS, lipopolysaccharide; TMT, tandem mass tag.