Figure 2.

Supplementation of macrophages with Se effects global proteomic changes upon treatment with LPS. BMDMs were stimulated with LPS for indicated time periods up to a maximum of 20 h. Cells were harvested and lysed to extract the proteome. Proteomic analysis is described in Experimental procedures. BMDMs cultured in Se-deficient media and not subjected to any stimulation were used for comparison. Data were normalized to β-actin, and abundance values are expressed as relative to zero-hour zero-Se cells (naïve cells). A, scatterplots show biological replicates after LPS, which are well correlated (Pearson’s r of 0.96). B, the bar chart shows the overall proteome changes during after LPS stimulation. Values are represented as log2 fold changes. C, the heat map showing the modulation of global BMDM proteome after LPS stimulation. D, the t-SNE analysis and k-means clustering of proteomic changes after LPS show seven distinct clusters, respectively. BMDMs, bone marrow–derived macrophages; LPS, lipopolysaccharide; Se, selenium; t-SNE, t-distributed Stochastic neighbor embedding.