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. 2021 Feb 11;296:100410. doi: 10.1016/j.jbc.2021.100410

Figure 7.

Figure 7

Selenoprotein-dependent changes in Shpk (Carkl).A and B, western immunoblot of Carkl expression upon stimulation of BMDMs with LPS in the presence or absence of Se. BMDMs were isolated from WT and Trspfl/flLysMCre mice, respectively, and cells were stimulated with 100-ng LPS on day eight for 1 h and harvested at 4, 8, and 20 h, followed by protein extraction and Western blot analysis. C and D, Densitometric analysis using ImageJ of Shpk (Carkl) expression that was normalized to the expression of β-actin. Shpk fold change in BMDMs incubated with Se were compared with those without Se. E, fold change in relative expression of Shpk in WT and LysM KO over time post LPS stimulation. Representative data shown are the mean ± SEM of n =3 per genotype at each time point after LPS stimulation. ∗p < 0.05. BMDMs, bone marrow–derived macrophages; LPS, lipopolysaccharide; Se, selenium.