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. 2021 Feb 11;296:100414. doi: 10.1016/j.jbc.2021.100414

Figure 7.

Figure 7

The ceRNA network of regulating NOD1 is widely found in teleost fish.A, sequence alignment of pre-miR-217 from various vertebrate species. Mature miR-217 sequences are shown in boxes. B, putative miR-217-5p-binding site of NOD1 3’UTR among different vertebrate species. C, putative miR-217-5p-binding site of NARL among different vertebrate species. D, the relative luciferase activities were detected in EPC cells after cotransfected with Lcr-NARL-wt or Lcr-NARL-mut and miR-217-5p or NC (upper panel), and cells after cotransfected with Ndi-NARL-wt or Ndi-NARL-mut and miR-217-5p or NC (lower panel). E, the relative luciferase activities were detected in EPC cells after cotransfected with Lcr-NOD1-wt or Lcr-NOD1-mut and miR-217-5p or NC (upper panel) and in cells after cotransfected with Ndi-NOD1-wt or Ndi-NOD1-mut and miR-217-5p or NC (lower panel). F, the relative luciferase activities were detected in EPC cells after cotransfection with Lcr-NOD1- 3’UTR luciferase reporter vector, NC, miR-217-5p, or Lcr-NARL (upper panel), and EPC cells after cotransfection with Ndi-NOD1-3’UTR luciferase reporter vector, NC, miR-217-5p, or Ndi-NARL (lower panel). All data represented the mean ± SD from three independent triplicated experiments. ∗p < 0.05; ∗∗p < 0.01.