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. 2021 Feb 25;24(3):102231. doi: 10.1016/j.isci.2021.102231

Figure 3.

Figure 3

The rad52-3KR mutation impairs Rad51-dependent type I survivor formation

(A) Mean replicative senescence curves of est2Δ, est2Δ rad51Δ (n = 4), and est2Δ rad51Δ rad52-3KR (n = 3) cells. Bars are SD.

(B) Replicative senescence est2Δ, est2Δ rad59Δ (n = 10), and est2Δ rad59Δ rad52-3KR (n = 8) clones. Bars are SD.

(C) The crisis period is determined as the number of days the cell population stays arrested for each individual clones analyzed. Crisis period average calculated for each mutant is represented. T test analysis between crisis period of est2Δ rad59Δ and est2Δ rad59Δ rad52-3KR spores scores 0.029 (∗).

(D) Cell density at crisis corresponds to the optical density (OD) at 600nm measured at the nadir of crisis. Average of the lowest OD600 during replicative senescence is represented for each mutant. T test analysis between est2Δ rad59Δ and est2Δ rad59Δ rad52-3KR spores scores 0.016 (∗).