Figure 6.

Rad59, but not Rad51, is required to tether eroded telomeres at the NPC

(A) Co-localization of Cdc13-YFP/Rad52-RFP foci with CFP-Nup49 during replicative senescence in est2Δ cells. Eroded telomeres in est2Δ cells are detected as foci containing both Rad52-RFP and Cdc13-YFP in the nup133ΔN background, which causes Nup49-CFP (NPCs) to cluster at one side of the nucleus. Representative images illustrating the colocalization of Cdc13, Rad51, and Nup49 are shown.

(B) Quantification of the triple colocalization of Cdc13-YFP, Rad52-RFP, and Nup49-CFP during senescence and at the time of crisis. The data are represented as the means ± SEM for 4, 2, 4, 2, and 2 biological replicates for the est2Δ, est2 rfa1-5KR, est2Δ rad51Δ, est2Δ rad59-2KR, and est2Δ rad59Δ mutants, respectively. Control est2Δ are from Churikov et al., (2016).