Table 2.
Validation of SSU using Canadian and Swedish isolates.
| Sample origin | Number of samples | Number (%) contigs produced | Number (%) called correctly | Number (%) called incorrectly | Number (%) flagged requiring manual analysis | Number (%) flagged in agreement with manual analysis | Number (%) flagged that are further resolved with manual analysis | Number (%) samples that a contig was unable to be produced | Number (%) non-contig samples in agreement with manual analysis in one or both directions | Number (%) non-contig samples not in agreement with manual analysis in one or both directions |
|---|---|---|---|---|---|---|---|---|---|---|
| Canada: | ||||||||||
| Human | 64 | 64 (100%) | 64 (100%) | 0 (0%) | 0 (0%) | 0 (0%) | 0 (0%) | 0 (0%) | 0 (0%) | 0 (0%) |
| Animala | 67b | 67 (100%) | 66 (98.5%) | 0 (0%) | 1 (1.5%) | 1 (100%) | 0 (0%) | 0 (0%) | 0 (0%) | 0 (0%) |
| Water | 133c | 129 (97.0%) | 129 (100%) | 0 (0%) | 0 (0%) | 0 (0%) | 0 (0%) | 4 (3.0%) | 4 (100%) | 0 (0%) |
| Sweden: | ||||||||||
| Animald | 167 | 157 (94.0%) | 154 (98.1%) | 2 (1.3%)e | 1 (0.6%) | 1 (100%) | 0 (0%) | 10 (6.0%) | 9 (90%) | 1 (10%)f |
| Total: | 431 | 417 (96.8%) | 413 (99.0%) | 2 (1.0%) | 2 (0.5%) | 2 (0.5%) | 0 (0%) | 14 (3.2%) | 13 (92.9%) | 1 (7.1%) |
a
Animal sample sources from 43 cattle, 20 dogs, 1 cat, 1 chicken, 2 snakes.
b
1 sample was not typable either by CryptoGenotyper or manually so have been removed from this total.
c
10 samples were not typable either by CryptoGenotyper or manually so have been removed from this total.
d
Animal sample sources from 117 cattle, 34 pigs, 10 squirrels, 3 sheep, 2 horses, 1 roe deer.
e
2 samples were called as C. parvum (Type A) and C. parvum (Type B) although this could not be confirmed in manual analysis.
f
One sample called as mixed infection in the reverse sequence although only one species could be identified in manual analysis.